Firefly & Renilla Luciferase Single Tube Assay Kit (zestaw do oznaczania lucyferazy Świetlika i Renilla w jednej probówce)

2 065,00 

cena PLN netto bez kosztów transportu

Nr kat. CA141

 

Opis

Sensitive dual-luciferase assay kit for Firefly and Renilla in a single tube.

The Firefly & Renilla Luciferase Single Tube Assay Kit enables sensitive, accurate, and fast measurement of dual luciferase activities in a single protein extract. This kit exploits the distinct biochemical requirements of Firefly (Photinus pyralis) and Renilla (Renilla reniformis) luciferases, making it highly effective for dual-reporter gene assays.

Its single-tube format ensures convenience, reducing handling errors and improving reproducibility. With enhanced sensitivity and linearity, the Firefly & Renilla Luciferase Single Tube Assay Kit is ideal for transcriptional regulation studies, gene expression analysis, and high-throughput screening.

Advantages & Features
  • Enhanced sensitivity: optimized for accurate dual luciferase detection.

  • Convenient format: single-tube workflow reduces variability.

  • Fast results: obtain luminescence readings within minutes.

  • High reproducibility: consistent batch-to-batch performance.

  • HTS-ready: suitable for high-throughput gene reporter assays.

Includes

– 10 mL Cell Lysis Buffer (5x)
– 10 mL Fluc Assay Buffer
– 10 mL Rluc Assay Buffer
– 2 x 1 mg D-Luciferin, potassium salt
– 2 x 200 μg Coelenterazine (lyophilized)

Documentation

CA141_Datasheet

updated MSDS upon request

Applications & Quality Control
  • Dual-reporter gene assays.

  • Transcriptional regulation studies.

  • Gene expression analysis.

  • High-throughput screening.

Quality Control

  • Each batch is validated for sensitivity, linearity, and reproducibility in Firefly and Renilla luminescence detection.

 

Advice & Storage & Shipping
  • Use fresh reagents and avoid repeated freeze-thaw cycles.

  • Protect assay components from light.

  • Handle luciferase substrates carefully to avoid degradation.

Storage, Shipping & Guarantee

  • Shipment at Dry Ice.
  • Storage: -80°C or below. Fluc and Rluc Assay Buffers are stable at -80°C for at least three months from date of receipt. Other components are stable at -20°C or below for at least three months from date of receipt. Kit components are stable to at least 5 freeze/thaw cycles.