FDG – Galactosidase Assay Kit (zestaw do oznaczania beta-galaktozydazy – FDG)
1 604,00 zł
cena PLN netto bez kosztów transportu
Nr kat. CA083
Fluorometric assay kit for sensitive detection of β-galactosidase activity in cells.
FDG β-Galactosidase Assay Kit is a fluorometric assay for measuring β-galactosidase activity in living cells. It uses fluorescein di-β-D-galactopyranoside (FDG) as a substrate that produces a fluorescent signal upon cleavage.
This kit provides sensitive and quantitative detection of enzyme activity and is suitable for reporter gene studies and cell-based assays.
High sensitivity: femtogram-level detection.
Convenient: ready-to-use reagents and buffers.
Robust: optimized for transfected cell lysates.
Fluorometric detection: Ex/Em = 490/525 nm.
Large capacity: 500 assays per kit.
| Specification | Description |
|---|---|
| Detection | Fluorometric (Ex/Em = 490/525 nm) |
| Sensitivity | Femtogram-level β-galactosidase |
| Assay type | Cell lysates (transfected with lacZ) |
| Assay capacity | 500 assays |
| Includes | Buffers, enzyme, FDG substrate, controls |
Includes
– 55 mL Reaction Buffer
– 25 mL Lysis Buffer
– 25 mL Stop solution
– 20 μL β-galactosidase enzyme (0.1 mg/mL)
– 500 μL β-Mercaptoethanol
– 150 μL FDG solution (10 mM)
Reporter gene assays (lacZ).
Measurement of β-galactosidase activity in transfected cell lysates.
Transfection efficiency monitoring.
Gene expression analysis.
Quality Control
Each batch validated for reproducibility and sensitivity.
Strict lot-to-lot quality assurance testing.
Protect FDG substrate from light.
Ensure complete lysis of cells for maximum signal.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: Upon receipt, store the kit at -20 ºC. β-galactosidase substrate must be protected from light.
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